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Detection of chondroitin sulfate proteoglycan 4 (CSPG4) in melanoma
Yangyang Wang1, Francesco Sabbatino, Xinhui Wang
1Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 22, 2013
Abstract:
The tumor antigen chondroitin sulfate proteoglycan 4 (CSPG4) appears to be a useful biomarker to identify melanoma cells and an attractive target to apply antibody-based immunotherapy for the treatment of melanoma. Here we described the reverse transcription-polymerase chain reaction (RT-PCR) method and the immunohistochemical (IHC) staining method to detect the expression of CSPG4 in melanoma cells and tissues.
