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Immune system functional pathway analysis using single cell network profiling (SCNP): a novel tool in cancer
Alessandra Cesano1, David Spellmeyer
1Nodality, Inc., South San Francisco, CA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 22, 2013
Summary
Novel biomarkers are crucial for cancer immunotherapy response. Single cell network profiling (SCNP) measures immune cell function, providing a systems-level view to identify responsive patients.
Area of Science:
- Immunology
- Biomarker Discovery
- Cancer Research
Background:
- Cancer immunotherapies have limited success, necessitating new biomarkers.
- A systems-level approach is needed to understand complex immune responses.
- Identifying patients likely to respond to immunotherapy remains a challenge.
Purpose of the Study:
- To introduce Single Cell Network Profiling (SCNP) as a novel method for immune function assessment.
- To detail the SCNP assay for measuring immune response at a systems level.
- To demonstrate the utility of SCNP for immune monitoring in cancer therapy.
Main Methods:
- SCNP is a multiparametric flow cytometry assay.
- It simultaneously measures extracellular markers and intracellular signaling proteins.
- Functional capacity is assessed by measuring signaling changes after external modulation.
Main Results:
- SCNP provides a systems-level assessment of immune function and dysfunction.
- Simultaneous analysis reveals connectivity within cell signaling networks and immune cell subtypes.
- The assay involves pre-analytical preparation, modulation, staining, data acquisition, and analysis.
Conclusions:
- SCNP offers a comprehensive method for immune monitoring.
- This approach can identify patients likely to benefit from cancer immunotherapies.
- Understanding immune network connectivity is key for therapeutic advancements.
