In vitro haploid plantlet induction in Physalis ixocarpa brot. through microspore embryogenesis
1Max-Planck-Institut für Züchtungsforschung (Erwin-Baur-Institut), D-5000, Köln 30, Federal Republic of Germany.
Plant Cell Reports
|November 22, 2013
Abstract:
Anthers of Physalis ixocarpa Brot. exised from 2-3 mm long flower buds were treated for 2 d at 3°C, and were cultured on the basal medium of NN (1969), supplemented with plant hormones. They formed embryoids from microspores within 6 weeks. Upon transfer to a regeneration medium, embryoids grew into functional plants with the haploid number of chromosomes (n=12).
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