Related Experiment Video

Updated: May 5, 2026

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
08:55

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

11.0K

Imaging the glycosylation state of cell surface glycoproteins by two-photon fluorescence lifetime imaging microscopy

Brian Belardi1, Adam de la Zerda, David R Spiciarich

  • 1Departments of Chemistry and Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, CA 94720 (USA).

Angewandte Chemie (International Ed. in English)
|November 22, 2013
PubMed
Abstract

No abstract available in PubMed .

Keywords:
bioorthogonal chemistryfluorescenceglycosylationmetabolic incorporationprostate cancer

More Related Videos

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

27.6K
Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
10:41

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

7.9K

Related Experiment Videos

Last Updated: May 5, 2026

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
08:55

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

11.0K
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

27.6K
Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
10:41

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

7.9K

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

1.9K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
1.9K

Articles linked to this work by shared authors, journal, and citation graph.

Insertion-Ligation Reconstitution of Transmembrane Proteins for Functional Interfaces in Synthetic Cells.

Nano letters·2026

AAV delivered lysosome-targeting chimeras mediate sustained antibody depletion in vivo.

bioRxiv : the preprint server for biology·2026

Jammed interconnected bilayer emulsions as 3D-printable biological tissue mimics.

Nature materials·2026

Tumor immune cell targeting chimeras reprogram tumor-associated macrophages.

Nature chemical biology·2026

Genetically targeted photocatalytic organic dyes for spatiotemporally controlled organic synthesis in specific living cells.

Nature chemistry·2026

Iterative Bump-and-Hole Engineering Creates a Bioorthogonal Reporter for N-Acetylglucosaminyltransferase I.

Journal of the American Chemical Society·2026

Tunable Raman Scattering of Fe(II) Coordination Networks With Magnetic Fields.

Angewandte Chemie (International ed. in English)·2026

Programmable Molecular Hubs Orchestrate Orthogonal CO2-to-CH4 Photocatalysis and Biomass Valorization.

Angewandte Chemie (International ed. in English)·2026

Inducing Radicality in Gold-Coordinated Porphyrinoids Through Precise Tip-Induced Covalent Bond Dissociation.

Angewandte Chemie (International ed. in English)·2026

Bench-Stable α-Fluoroamines: Synthesis, Properties, and Application in Medicinal Chemistry.

Angewandte Chemie (International ed. in English)·2026

Using Aromatic-Bridge-Tuned Dimer Acceptors to Harmonize Photoluminescence Quantum Yield and Crystallinity for Organic Solar Cells Exceeding 21% Efficiency.

Angewandte Chemie (International ed. in English)·2026

N-Heterocyclic Carbene-Triarylamine Hybrids as Redox-Tunable Organophotocatalysts for Radical Cross-Coupling Reactions.

Angewandte Chemie (International ed. in English)·2026

The Individual in Medicine: Why Anomalies Matter.

Journal of evaluation in clinical practice·2026

Reclassification of Noncanonical MET Splice-Site Variants Using RNA-Based Validation: A Practical Diagnostic Approach in Non-Small Cell Lung Cancer.

Turk patoloji dergisi·2026

Evolutionary and structural pathways of antibody maturation reveal principles for engineering potent SARS-CoV-2 neutralizers.

Journal of structural biology: X·2026

High-Throughput DNA Barcoding Identifies Lipid Nanoparticles for Lung Delivery of TRAIL-mRNA to Induce Tumor Cell Death in Experimental Metastasis Model.

International journal of nanomedicine·2026

Segmented poly(A) tails with microRNA target sites confer tissue-specific regulation for mRNA therapeutics.

Molecular therapy. Nucleic acids·2026

TGF-β-induced epithelial to mesenchymal transition drives proteoglycan binding specificity of HPV-based drug conjugates for cancer cells.

Frontiers in oncology·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us