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Updated: May 5, 2026
A Simplified Method for Agrobacterium-mediated Transformation of Phytophthora palmivora
Published on: March 20, 2026
AgarTrap: a simplified Agrobacterium-mediated transformation method for sporelings of the liverwort Marchantia
Shoko Tsuboyama1, Yutaka Kodama
1Center for Bioscience Research and Education, Utsunomiya University, Tochigi, 321-8505 Japan.
Abstract:
The liverwort Marchantia polymorpha L. is being developed as an emerging model plant, and several transformation techniques were recently reported. Examples are biolistic- and Agrobacterium-mediated transformation methods. Here, we report a simplified method for Agrobacterium-mediated transformation of sporelings, and it is termed Agar-utilized Transformation with Pouring Solutions (AgarTrap). The procedure of the AgarTrap was carried out by simply exchanging appropriate solutions in a Petri dish, and completed within a week, successfully yielding sufficient numbers of independent transformants for molecular analysis (e.g. characterization of gene/protein function) in a single experiment. The AgarTrap method will promote future molecular biological study in M. polymorpha.
