Related Experiment Video
Updated: May 5, 2026

Author Spotlight: Enhancing PSC-to-Functional Cell Differentiation Using ML Models Based on Live-Cell Bright-Field Imaging
Published on: October 4, 2024
Differentiation-defective phenotypes revealed by large-scale analyses of human pluripotent stem cells
Michiyo Koyanagi-Aoi1, Mari Ohnuki, Kazutoshi Takahashi
1Center for iPS Cell Research and Application, Department of Biological Repair, Institute for Frontier Medical Sciences, and Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto 606-8507, Japan.
Abstract:
We examined the gene expression and DNA methylation of 49 human induced pluripotent stem cells (hiPSCs) and 10 human embryonic stem cells and found overlapped variations in gene expression and DNA methylation in the two types of human pluripotent stem cell lines. Comparisons of the in vitro neural differentiation of 40 hiPSCs and 10 human embryonic stem cells showed that seven hiPSC clones retained a significant number of undifferentiated cells even after neural differentiation culture and formed teratoma when transplanted into mouse brains. These differentiation-defective hiPSC clones were marked by higher expression levels of several genes, including those expressed from long terminal repeats of specific human endogenous retroviruses. These data demonstrated a subset of hiPSC lines that have aberrant gene expression and defective potential in neural differentiation, which need to be identified and eliminated before applications in regenerative medicine.
More Related Videos
09:07Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency
Published on: June 10, 2018
14:37Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
Published on: November 1, 2017
Related Concept Videos
Stem Cell Culture
iPS Cell Differentiation
Induced Pluripotent Stem Cells
Somatic...
Induced Pluripotent Stem Cells