Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Action potentials in human macrophages are calcium spikes.

H J Woehlck, F V McCann

    Cell Biology International Reports
    |July 1, 1986
    PubMed
    Summary

    Human macrophages generate action potentials dependent on calcium, not sodium. This finding is crucial for understanding macrophage electrical activity and potential therapeutic targets.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Impact of a Transplantation Critical Care Model on Short-Term Outcomes Following Liver Transplantation in High Acuity Patients: A Single-Center Experience.

    Transplantation proceedings·2018
    Same author

    Rainbow after the storm.

    British journal of anaesthesia·2017
    Same author

    Angiotensin-receptor-blocker-induced refractory hypotension responds to methylene blue.

    Acta anaesthesiologica Scandinavica·2012
    Same author

    CO2 chemosensitivity in Helix aspersa: three potassium currents mediate pH-sensitive neuronal spike timing.

    American journal of physiology. Cell physiology·2006
    Same author

    Carbon monoxide rebreathing during low flow anesthesia.

    Anesthesia and analgesia·2001
    Same author

    Increased carboxyhemoglobin from hemolysis mistaken as intraoperative desflurane breakdown.

    Anesthesia and analgesia·2001

    Area of Science:

    • Cellular Electrophysiology
    • Immunology
    • Membrane Biology

    Background:

    • Macrophages play critical roles in immune responses.
    • The electrical properties of macrophages, particularly their action potentials, are not fully understood.
    • Investigating the ion channel mechanisms underlying macrophage excitability is essential.

    Purpose of the Study:

    • To investigate the ionic basis of action potential generation in human monocyte-derived macrophages.
    • To determine the role of sodium and calcium ions in macrophage electrophysiological activity.

    Main Methods:

    • Human monocyte-derived macrophages were utilized.
    • Transmembrane resting and action potentials were recorded using single and double microelectrode impalements.
    • Depolarizing current pulses were applied to induce repetitive action potentials.

    Main Results:

    • Repetitive action potentials in macrophages were found to be sodium-insensitive.
    • Action potential generation was confirmed to be calcium-dependent.
    • Extracellular sodium levels, choline substitution, and tetrodotoxin did not affect action potential characteristics; however, calcium levels and blockers like cobalt and verapamil significantly altered spiking activity.

    Conclusions:

    • Macrophage action potentials are primarily mediated by calcium influx, not sodium.
    • These findings highlight a novel electrophysiological mechanism in macrophages.
    • Understanding calcium-dependent action potentials in macrophages may offer new therapeutic avenues.

    Related Experiment Videos