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Related Concept Videos

Fractures: Bone Repair01:27

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Treatment for a fracture is based on the type of break, the bone affected, and the patient's age.
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The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
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The minerals contained in all of the food we consume are essential for our organ systems. However, certain essential minerals, such as calcium, phosphorus, magnesium, manganese, and fluoride, largely affect bone health.
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Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
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Chondrocytes form a temporary cartilaginous model by dividing and secreting a thick gel-like extracellular matrix. Once the chondrocytes undergo programmed cell death, osteoblasts enter the site of the cartilaginous model. The process of replacing the temporary cartilaginous model with bone in an ordered manner is called endochondral ossification. In endochondral ossification, not all of the cartilage is replaced by bone tissue. Some cartilage that performs a protective and supportive function...
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Real-Time Imaging of CCL5-Induced Migration of Periosteal Skeletal Stem Cells in Mice
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Complement C3 and C5 deficiency affects fracture healing.

Christian Ehrnthaller1, Markus Huber-Lang, Per Nilsson

  • 1Department of Traumatology, Hand-, Plastic-, and Reconstructive Surgery, Center of Surgery, University of Ulm, Ulm, Germany.

Plos One
|November 22, 2013
PubMed
Summary
This summary is machine-generated.

The terminal complement cascade, particularly C5, is crucial for effective fracture healing. While C3 deficiency shows early deficits, C5 deficiency significantly impairs bone repair and strength.

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Area of Science:

  • Immunology
  • Orthopedics
  • Biomedical Engineering

Background:

  • Complement system activation is implicated in bone development and repair.
  • The complement receptor C5aR is expressed in fracture calluses by immune and bone cells, suggesting a role in healing.
  • The specific roles of complement components C3 and C5 in fracture healing remain unclear.

Purpose of the Study:

  • To investigate the role of the complement system, specifically C3 and C5, in murine fracture healing.
  • To elucidate the impact of C3 and C5 deficiency on the early and late stages of bone repair.

Main Methods:

  • Standardized femur osteotomies were performed on C3(-/-), C5(-/-), and wildtype mice.
  • Fracture healing was assessed at 7 and 21 days post-injury.
  • Histological, micro-computed tomography (micro-CT), and biomechanical analyses were employed.

Main Results:

  • Both C3(-/-) and C5(-/-) mice exhibited reduced callus area and newly formed bone in the early healing phase.
  • While C3 deficiency did not prevent eventual successful healing, C5 deficiency led to significantly impaired fracture repair at 21 days.
  • C5-deficient mice showed reduced bending stiffness and callus volume, indicating compromised biomechanical stability.

Conclusions:

  • The terminal complement cascade, especially involving C5, plays a critical role in successful fracture healing.
  • C5 activation appears essential for achieving adequate bone repair and biomechanical strength.
  • Complement system modulation may offer therapeutic targets for enhancing bone healing.