Related Experiment Video
Updated: May 5, 2026

Surface Spreading and Immunostaining of Yeast Chromosomes
Published on: August 9, 2015
New method for visualization of C-heterochromatin in synaptonemal complex spreads
1Institute of Cytology and Genetics,Russian Academy of Sciences, Siberian Department & Novosibirsk State University, Novosibirsk 630090, Russia.
Abstract:
DAPI staining of the metaphase chromosomes pretreated with barium hydroxide generates a C-like banding pattern. In this work a protocol for visualizing similar pattern at the synaptonemal complex (SC) spreads after immunostaining is suggested. This method was used to visualize centromeric and sex heterochromatin at the SC spreads of guppy fish (Poecilia reticulata Peters, 1859). The efficiency of this method was further confirmed at SC spreads of the northern red-backed vole (Myodes rutilus (Pallas, 1779)), the guinea pig (Cavia porcellus (Linnaeus, 1758)), and the pigmy shrew (Sorex minutus Linnaeus, 1766).
Related Concept Videos
Spreading of Chromatin Modifications
Writers
The writer...
Heterochromatin
Heterochromatin
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
FISH - Fluorescent In-situ Hybridization
Duplication of Chromatin Structure
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...

