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Updated: May 5, 2026
Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
Published on: September 20, 2019
CD24(hi)CD27(+) B cells from patients with allergic asthma have impaired regulatory activity in response to
L E P M van der Vlugt1, E Mlejnek, A Ozir-Fazalalikhan
1Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.
Background:
Regulatory B cells have been identified that strongly reduce allergic and auto-immune inflammation in experimental models by producing IL-10. Recently, several human regulatory B-cell subsets with an impaired function in auto-immunity have been described, but there is no information on regulatory B cells in allergic asthma.
Objective:
In this study, the frequency and function of IL-10 producing B-cell subsets in allergic asthma were investigated.
Methods:
Isolated peripheral blood B cells from 13 patients with allergic asthma and matched healthy controls were analyzed for the expression of different regulatory B-cell markers. Next, the B cells were activated by lipopolysaccharide (LPS), CpG or through the B-cell receptor, followed by co-culture with endogenous memory CD4(+) T cells and house dust mite allergen DerP1.
Results:
Lower number of IL-10 producing B cells were found in patients in response to LPS, however, this was not the case when B cells were activated through the B-cell receptor or by CpG. Further dissection showed that only the CD24(hi)CD27(+) B-cell subset was reduced in number and IL-10 production to LPS. In response to DerP1, CD4(+) T cells from patients co-cultured with LPS-primed total B cells produced less IL-10 compared to similar cultures from controls. These results are in line with the finding that sorted CD24(hi)CD27(+) B cells are responsible for the induction of IL-10(+) CD4(+) T cells.
Conclusions:
Taken together, these data indicate that CD24(hi)CD27(+) B cells from allergic asthma patients produce less IL-10 in response to LPS leading to a weaker IL-10 induction in T cells in response to DerP1, which may play a role in allergic asthma.
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