Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

5.0K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-World Emulation of Landmark Lung Cancer Trials: A Registry-Based Reconstruction of KN189, KN407, IMpower133, and PACIFIC.

Cancers·2026
Same author

Sebaceous Carcinoma: A Retrospective Multicenter Analysis of 213 Cases.

Cancers·2026
Same author

Use of bevacizumab for patients with International Federation of Gynecology and Obstetrics stage IIIB to IV epithelial ovarian cancer undergoing primary debulking surgery and its association with oncologic outcomes: a Cancer Registry study.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society·2025
Same author

A comparative computational analysis of IFN-alpha pharmacokinetics and its induced cellular response in mice and humans.

PLoS computational biology·2025
Same author

Differential regulation of calcium-activated plant kinases in Arabidopsis thaliana.

The Plant journal : for cell and molecular biology·2025
Same author

YAP charge patterning mediates signal integration through transcriptional co-condensates.

Nature communications·2025

Related Experiment Video

Updated: May 5, 2026

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils
09:44

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils

Published on: January 3, 2025

1.3K

Ca2+ dynamics correlates with phenotype and function in primary human neutrophils.

Katrin Hübner1, Irina Surovtsova, Klaus Yserentant

  • 1Modeling of Biological Processes, BioQuant, COS Heidelberg, University of Heidelberg, Heidelberg, Germany.

Biophysical Chemistry
|November 23, 2013
PubMed
Summary

This study reveals that specific calcium dynamics in neutrophils correlate with their functional states and phenotypes. These calcium signals appear to guide neutrophils through spontaneous changes between cellular states.

Keywords:
Calcium signalingMultivariate live cell imagingNeutrophilOscillationPlasticity

More Related Videos

Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
09:39

Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils

Published on: June 2, 2023

2.7K
Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy
10:40

Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy

Published on: July 17, 2013

12.8K

Related Experiment Videos

Last Updated: May 5, 2026

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils
09:44

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils

Published on: January 3, 2025

1.3K
Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
09:39

Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils

Published on: June 2, 2023

2.7K
Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy
10:40

Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy

Published on: July 17, 2013

12.8K

Area of Science:

  • Immunology
  • Cell Biology
  • Biophysics

Background:

  • Neutrophils are critical immune cells with functions including sensing, motility, and killing.
  • Neutrophil populations exhibit heterogeneity in cellular states, maturity, and phenotypic plasticity.
  • Intracellular calcium dynamics are known to be vital for neutrophil functions like motility.

Purpose of the Study:

  • To characterize cell-to-cell variability in neutrophil morphology and calcium dynamics.
  • To identify distinct neutrophil phenotypes and their distribution.
  • To investigate the relationship between intracellular calcium responses and morphological changes.

Main Methods:

  • Long-term multivariate live cell imaging of single primary human neutrophils.
  • Quantitative analysis of cell morphology and motility parameters.
  • Simultaneous measurement of intracellular calcium dynamics and shape changes.

Main Results:

  • Five distinct neutrophil subpopulations were identified based on morphology and motility.
  • Individual neutrophil calcium dynamics were found to correlate significantly with their functional state.
  • Spontaneous phenotypic transitions between cellular states were observed, often preceded or accompanied by specific calcium dynamics.

Conclusions:

  • Specific intracellular calcium dynamics convey critical information regarding neutrophil function and phenotype.
  • Neutrophil heterogeneity and plasticity are influenced by distinct calcium signaling patterns.
  • Understanding neutrophil calcium dynamics provides insights into immune defense mechanisms.