Vascularisation is not necessary for gut colonisation by enteric neural crest cells
Jean-Marie Delalande1, Dipa Natarajan1, Bertrand Vernay1
1Neural Development Unit, UCL Institute of Child Health, 30 Guilford Street, London WC1N 1EH, United Kingdom.
Developmental Biology
|November 23, 2013
Summary
Blood vessels do not guide enteric neural crest cell migration during enteric nervous system development. Both the vascular and nervous systems develop independently in the developing gut.
Area of Science:
- Developmental biology
- Neuroscience
- Vascular biology
Background:
- The enteric nervous system (ENS) and vasculature share similar architectures.
- Previous hypotheses suggested vascular networks guide enteric neural crest cells (ENCC) migration for ENS formation.
Purpose of the Study:
- To investigate the relationship between ENCC migration, ENS formation, and gut vascular development.
- To determine if vascular networks are essential for guiding ENCC during gut colonization.
Main Methods:
- Fate-mapping of ENCC
- Immunolabeling
- Intravascular dye injection
- Genetic manipulation of vascular development (Vegfa(120/120), Tie2-Cre;Nrp1(fl/-) mice)
- In vitro ENS development models (Wnt1-Cre;Rosa26(Yfp/+))
Main Results:
- Enteric and vascular networks exhibit distinct developmental patterns.
- ENCC migration occurs in vascular-poor foregut regions.
- ENCC distribution in vessel-rich midgut and hindgut does not follow vascular patterns.
- ENCC colonize the entire gut even with impaired vascular development.
- Hindgut vascular development is normal in ENS-deficient (miRet(51)) mice.
Conclusions:
- Gut vascular networks are not necessary for guiding ENCC migration during ENS development.
- Enteric and vascular network development are largely independent processes in the early gut.
Related Concept Videos
01:26Enteral Nutrition I: Orogastric and Nasogastric Feeding
1.5K
Enteral nutrition delivers nutrients directly to the stomach or small intestine through a tube. This method is appropriate for patients who cannot eat but still have a functioning digestive system. It is also beneficial for individuals with swallowing difficulties, anorexia, malabsorption, or those who have undergone gastrointestinal (GI) surgery.
Orogastric (OG) and nasogastric (NG) feeding are two standard methods used for enteral nutrition. Enteral nutrition is often preferred over...
Orogastric (OG) and nasogastric (NG) feeding are two standard methods used for enteral nutrition. Enteral nutrition is often preferred over...
1.5K
01:05Physiology of Enteric Nervous System and Gut Health
1.0K
The gastrointestinal tract, responsible for the digestion and absorption of nutrients, is safeguarded by the intestinal barrier, which consists of secretory, physical, and immune components. At the forefront is the secretory barrier, composed of essential elements such as mucus, gut microbiota, and defense proteins. They collaborate to break down food particles, facilitate nutrient absorption, and maintain optimal gut health. These secretory components ensure the smooth functioning of the...
1.0K
01:37Neural Regulation
43.4K
Digestion begins with a cephalic phase that prepares the digestive system to receive food. When our brain processes visual or olfactory information about food, it triggers impulses in the cranial nerves innervating the salivary glands and stomach to prepare for food.
43.4K
01:18Drug Delivery: Enteral Route
1.8K
The enteral drug administration involves three primary routes: oral, sublingual, and buccal. Oral ingestion is the most prevalent, safe, economical, and convenient method for drug administration. However, it has certain drawbacks, including limited absorption due to the drug's low water solubility or poor membrane permeability, possible emesis from GI mucosa irritation, destruction of drugs by digestive enzymes or low gastric pH, and irregular absorption along with food or other drugs.
1.8K
01:18Routes of Drug Administration: Enteral
6.5K
Medications can be administered through the enteral route using liquids, capsules, or tablets.
Enteral administration involves drug administration via the mouth in two ways: orally or sublingually.
Unlike sublingually drugs, drugs that are taken orally pass through the gastrointestinal (GI) tract and get metabolized by the liver. Once metabolized, the drug is absorbed into the systemic circulation, reaching different body parts via the bloodstream. However, while passing through the stomach,...
Enteral administration involves drug administration via the mouth in two ways: orally or sublingually.
Unlike sublingually drugs, drugs that are taken orally pass through the gastrointestinal (GI) tract and get metabolized by the liver. Once metabolized, the drug is absorbed into the systemic circulation, reaching different body parts via the bloodstream. However, while passing through the stomach,...
6.5K
01:11Enteric Nervous System: Regulation of GI Motor Activity
1.9K
The Enteric Nervous System (ENS) plays a pivotal role in regulating gastrointestinal or GI motor activity. This complex network of nerves, deeply embedded within the gut wall, responds to changes in the gut environment and receives input from both the autonomic nervous system and the central nervous system. By doing so, the ENS operates various programs tailored to the body's nutritional status and needs.
During periods of fasting, the ENS initiates the migrating myoelectric complex, a...
During periods of fasting, the ENS initiates the migrating myoelectric complex, a...
1.9K