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Updated: May 5, 2026

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
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Clathrin and post-Golgi trafficking: a very complicated issue

David G Robinson1, Peter Pimpl2

  • 1Centre for Organismal Studies (COS), University of Heidelberg, Heidelberg, Germany.

Trends in Plant Science
|November 23, 2013
PubMed

Insights

Clathrin-coated vesicles (CCVs) assemble at the trans-Golgi network (TGN), but their function remains unclear. Studies on AP1M mutations do not confirm CCVs mediate vacuolar protein export from the TGN.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Protein Trafficking

Background:

  • Clathrin-coated vesicles (CCVs) are crucial for endocytosis at the plasma membrane.
  • CCVs also assemble at the trans-Golgi network (TGN), but their specific role there is not well understood.
  • Adaptor protein 1 (AP1) complexes, including the μ-adaptin subunit AP1M, are involved in TGN vesicle formation.

Purpose of the Study:

  • To investigate the function of CCVs at the trans-Golgi network (TGN).
  • To clarify the role of AP1M in vacuolar and secretory protein transport.
  • To determine if CCVs mediate receptor-mediated export of vacuolar proteins from the TGN.

Main Methods:

  • Analysis of knockout mutations in the AP1M subunit of adaptor protein 1.
  • Examination of vacuolar and secretory protein transport pathways.
  • Evaluation of potential roles for TGN maturation in protein transport.

Main Results:

  • AP1M mutations disrupt multiple TGN trafficking pathways.
  • These mutations do not provide conclusive evidence for CCVs mediating vacuolar protein export.
  • Alternative mechanisms, such as TGN maturation, could account for vacuolar protein transport.

Conclusions:

  • The precise function of CCVs at the TGN is still uncertain.
  • AP1M knockout studies are insufficient to establish CCVs as mediators of vacuolar protein export.
  • Vacuolar protein transport may occur via TGN maturation and subsequent fusion with the vacuole.
Keywords:
TGN maturationadaptor mutantsclathrin-coated vesiclesvacuolar protein transport

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