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Updated: May 5, 2026

Micropunching Lithography for Generating Micro- and Submicron-patterns on Polymer Substrates

Micropunching Lithography for Generating Micro- and Submicron-patterns on Polymer Substrates

Published on: July 2, 2012

Large-area molecular patterning with polymer pen lithography.

Daniel J Eichelsdoerfer¹, Xing Liao, Maria D Cabezas

¹Department of Chemistry, Northwestern University, Evanston, Illinois, USA.

Nature Protocols

|November 23, 2013

Summary

This summary is machine-generated.

Polymer pen lithography (PPL) enables massively parallel, sub-100-nm molecular printing for creating nanostructured surfaces. This technique is valuable for biological applications and high-throughput screening.

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Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies

Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies

Published on: June 12, 2018

Area of Science:

Biotechnology
Materials Science
Surface Chemistry

Background:

Constructing surfaces with nanostructured chemical functionality is crucial for biology and biotechnology.
Scanning probe techniques offer high resolution but often lack parallelization.

Purpose of the Study:

To describe a protocol for molecular printing using polymer pen lithography (PPL).
To demonstrate the fabrication of nanostructures for biological applications and high-throughput screening.

Main Methods:

Utilized polymer pen lithography (PPL), a cantilever-free scanning probe technique.
Fabricated lithographic apparatus and deposited alkanethiol and polymer on gold and silicon surfaces.
Generated nanostructures composed of proteins and metals.

Main Results:

Achieved sub-100-nm molecular feature generation in a massively parallel fashion.
Successfully created nanostructures using proteins and metals.
Demonstrated the creation of combinatorial arrays for high-throughput screening.

Conclusions:

PPL is an effective method for generating nanostructured surfaces with precise chemical functionality.
The protocol facilitates the creation of combinatorial arrays, enabling powerful high-throughput screening.
This technique supports diverse biologically relevant applications.