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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
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Trace elements in human livers using quality control in the complete analytical process.

R Zeisler1, S H Harrison, S A Wise

  • 1Center for Analytical Chemistry, National Bureau of Standards, 20234, Washington, DC.

Biological Trace Element Research
|November 23, 2013
PubMed
Summary
This summary is machine-generated.

Accurate trace element analysis in human livers is crucial for environmental and health studies. This program ensures data validity through rigorous quality control and multiple analytical techniques for reliable results.

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Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Toxicology

Background:

  • Data validity and intercomparability are critical for medical, environmental, and geochemical health research.
  • Establishing precise and accurate methods for trace constituent determination is essential.
  • The Environmental Protection Agency/National Bureau of Standards Pilot Environmental Specimen Bank Program addresses these needs.

Purpose of the Study:

  • To develop and validate an analytical approach for precise and accurate determination of trace constituents in human livers.
  • To ensure a valid relationship between analytical results and true sample values.
  • To minimize sample contamination and loss, and assure representative analytical portions.

Main Methods:

  • Analysis of human liver specimens under strict quality control.
  • Utilizing techniques such as atomic absorption spectrometry, isotope dilution mass spectrometry, neutron activation analysis, and voltammetry.
  • Verifying analytical technique accuracy using Standard Reference Materials and employing multiple independent techniques for element determination.

Main Results:

  • The program successfully analyzed 36 human livers in its first year.
  • Results were obtained for 31 elements, including selenium (Se) and lead (Pb).
  • Analytical methods were verified for accuracy and reliability.

Conclusions:

  • The implemented analytical approach ensures data validity and intercomparability for trace elements in human livers.
  • The program provides a reliable framework for monitoring environmental and health-related elements.
  • Rigorous quality control and multi-technique verification enhance the trustworthiness of the findings.