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Optimizing the Use of a Liquid Handling Robot to Conduct a High Throughput Forward Chemical Genetics Screen of Arabidopsis thaliana
Published on: April 30, 2018
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Studies on the use of toxic precursor analogs of opines to select transformed plant cells
1Institut de Microbiologie, Université de Paris-Sud, Bâtiment 409, F-91405, Orsay, France.
Abstract:
S-(2-aminoethyl-)L-cysteine and L-canavanine were less toxic for octopine-type crown gall tissues that contained lysopine dehydrogenase than for other crown gall or habituated tissues. These analogs are substrates for lysopine dehydrogenase in vitro and in vivo. Thus toxic analogs of amino acid precursors of opines may be useful in selecting for cells that contain an opine dehydrogenase.
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