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The influence of calcium and magnesium on manganese transport and utilization in Mice
A A Van Barneveld1, C J Van den Hamer
1Department of Nuclear Biotechnique, Interuniversity Reactor Institute, Mekelweg 15, 2629, JB Delft, The Netherlands.
Abstract:
The influence of high but not extreme concentrations of Ca and Mg in water (25 µmol/mL) and of similar concentrations in food (25 µmol/g) on the absorption and retention of(54)Mn in mice was investigated. Addition of either Ca or Mg reduced the absorption of(54)Mn from water (administered by intragastric intubation) and from food (a portion made available for consumption during 1.5 h) by more than 50%. The retention of intraperitoneally injected(54)Mn was not influenced by addition of Ca or Mg (25 µmol/mL) to the drinking water. In mice fed a Ca-depleted diet during 2 wk prior to dosage, the absorption of(54)Mn from an intubated aqueous solution was increased by more than 100% compared to that in mice fed a Ca-adequate diet. In mice fed a Mg-depleted diet or a Ca- or Mg-replete diet for 2 wk, no changes in the absorption of(54)Mn were observed. In mice fed a Ca,Mg-depleted diet after(54)Mn administration, the turnover of absorbed(54)Mn was strongly increased, probably as a result of increased absorption of stable Mn from the diet. The results indicate that the uptake of Mn could be higher in soft water regions than in hard water regions.
Insights
High calcium (Ca) and magnesium (Mg) in water and food significantly reduce manganese (Mn) absorption. Dietary Ca depletion increases Mn absorption, suggesting higher Mn uptake in soft water areas.
Area of Science:
- Environmental Science
- Toxicology
- Nutritional Science
Background:
- Calcium (Ca) and magnesium (Mg) are essential minerals found in water and food.
- Manganese (Mn) is an essential trace element crucial for various biological processes.
- Water hardness, influenced by Ca and Mg levels, may affect mineral absorption.
Purpose of the Study:
- To investigate the impact of high Ca and Mg concentrations on manganese (Mn) absorption and retention in mice.
- To determine how dietary Ca and Mg levels influence Mn uptake.
- To explore the relationship between water hardness and Mn bioavailability.
Main Methods:
- Mice were administered (54)Mn orally (intubation and food) and intraperitoneally.
- High concentrations of Ca and Mg were added to drinking water and food.
- Mice were fed Ca- or Mg-depleted diets prior to or after (54)Mn administration.
- Absorption and retention of (54)Mn were measured under different mineral intake conditions.
Main Results:
- Addition of Ca or Mg to water or food reduced (54)Mn absorption by over 50%.
- Intraperitoneal injection of (54)Mn showed no altered retention with added Ca or Mg in drinking water.
- Ca-depleted diets significantly increased (54)Mn absorption (>100%), while Mg-depleted diets did not.
- Depletion of both Ca and Mg post-administration increased (54)Mn turnover, likely due to enhanced stable Mn absorption.
Conclusions:
- High Ca and Mg concentrations in water and food inhibit Mn absorption.
- Dietary Ca availability plays a critical role in regulating Mn absorption.
- Mn uptake may be higher in soft water environments compared to hard water regions due to lower Ca and Mg levels.
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