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Updated: May 5, 2026

Single Nucleotide Polymorphism-sensitive FISH Detection of Locus-specific Ribosomal RNA Transcription in Drosophila melanogaster
Published on: March 28, 2025
Detection of two different nuclear genomes in parasexual hybrids by ribosomal RNA gene analysis
H Uchimiya1, T Ohgawara, H Kato
1Institute of Biological Sciences, University of Tsukuba, Sakura-mura, Japan.
Abstract:
Restriction endonucleases discriminated between the rDNAs contained in callus tissues derived from Nicotiana glauca, N. langsdorffii, and their somatic hybrids produced by protoplast fusion. With XbaI, a single repeat fragment of 7.5 × 10(6) daltons was produced from N. glauca rDNA compared to a single repeat fragment of 4.2 × 10(6) daltons produced from N. langsdorffii rDNA. Both kinds of XbaI fragments were found in somatic hybrids.
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