Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

SDS-PAGE

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...

Western Blotting

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Study of genetic determination of 20 gliadin bands.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

Same author

A comparison of androgenetic doubled-haploid, and single seed descent lines in Triticale.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

Same author

High molecular weight glutenin subunit in durum wheat (T. durum).

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

Same author

Selection indices for quality evaluation in wheat breeding.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

Same author

Polymorphism of omega-gliadins in durum wheat as revealed by the two-step APAGE/SDS-PAGE technique.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

Same author

Genetic characterization of storage proteins in a set of F1-derived haploid lines in bread wheat.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

Search research articles

Related Experiment Video

Updated: May 5, 2026

Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis

Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis

Published on: December 15, 2011

Correlation between gliadin bands.

¹Institut National de la Recherche Agronomique, Station d'Amélioration des Plantes, Clermont-Ferrand, France.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik

|November 23, 2013

Summary

This summary is machine-generated.

Researchers analyzed gliadin bands in bread wheat using starch gel electrophoresis. Positive correlations between bands suggest genes are located on the same chromosome, with linkage explaining most observed correlations.

More Related Videos

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation

Published on: November 26, 2018

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

Published on: July 5, 2022

Related Experiment Videos

Last Updated: May 5, 2026

Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis

Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis

Published on: December 15, 2011

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation

Published on: November 26, 2018

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

Published on: July 5, 2022

Area of Science:

Plant genetics
Molecular biology
Biochemistry

Background:

Gliadins are major storage proteins in wheat, contributing to dough quality.
Understanding gliadin variation is crucial for breeding programs.
Genetic linkage influences the inheritance of protein components.

Purpose of the Study:

To investigate correlations between gliadin bands observed via electrophoresis.
To determine if these correlations are due to genetic linkage.
To group correlated gliadin bands based on their co-inheritance patterns.

Main Methods:

Starch gel electrophoresis was performed on 37 diverse bread wheat cultivars.
41 gliadin bands were analyzed for simple correlations.
Genetic analysis of 312 F2 grains was conducted to study band distribution.

Main Results:

A significant negative correlation was observed between adjacent gliadin bands.
Positive correlations were found among 32 distinct groups of gliadin bands.
Two-thirds of the calculated correlations in progeny were attributed to genetic linkage.

Conclusions:

Correlated gliadin bands likely result from genes located on the same chromosome.
Genetic linkage is a primary driver of gliadin band co-inheritance.
This study provides insights into the genetic organization of gliadin genes in bread wheat.