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Updated: May 5, 2026
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
Published on: December 5, 2016
Method for site-specific detection of m6A nucleoside presence in RNA based on high-resolution melting (HRM) analysis
Anna Y Golovina1, Margarita M Dzama, Kirill S Petriukov
1Department of Chemistry, Lomonosov Moscow State University, Moscow 119992, Russia, Department of Bioinformatics and Bioengineering, Lomonosov Moscow State University, Moscow 119992, Russia and A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.
Abstract:
Chemical landscape of natural RNA species is decorated with the large number of modified nucleosides. Some of those could easily be detected by reverse transcription, while others permit only high-performance liquid chromatography or mass-spectrometry detection. Presence of m(6)A nucleoside at a particular position of long RNA molecule is challenging to observe. Here we report an easy and high-throughput method for detection of m(6)A nucleosides in RNA based on high-resolution melting analysis. The method relies on the previous knowledge of the modified nucleoside position at a particular place of RNA and allows rapid screening for conditions or genes necessary for formation of that modification.
