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Related Experiment Videos

Ion channels in yeast.

M C Gustin, B Martinac, Y Saimi

    Science (New York, N.Y.)
    |September 12, 1986
    PubMed
    Summary
    This summary is machine-generated.

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    Researchers identified voltage-dependent potassium channels in yeast. These channels activate with positive voltage, are selective for potassium, and exhibit specific conductance and inhibition properties.

    Area of Science:

    • Biophysics
    • Cell Biology
    • Molecular Biology

    Background:

    • Voltage-dependent ion channels are crucial for cellular function.
    • The presence and characteristics of these channels in yeast Saccharomyces cerevisiae are not fully understood.

    Purpose of the Study:

    • To investigate the presence and properties of voltage-dependent ion channels in the plasma membrane of Saccharomyces cerevisiae.

    Main Methods:

    • Utilized the patch-clamp technique to record ion channel activities.
    • Examined ion channel activities from spheroplasts and plasma membrane patches.

    Main Results:

    • Identified prominent potassium channel activity.
    • These channels exhibit voltage-dependent activation (positive voltages only).

    Related Experiment Videos

  • Demonstrated high selectivity for potassium over sodium ions, a unit conductance of 20 pS, inhibition by tetraethylammonium and barium ions, and bursting kinetics.

    • Conclusions:

    • Saccharomyces cerevisiae possesses voltage-dependent potassium channels.
    • These channels display unique properties distinct from previously characterized channels.