Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitochondria01:37

Mitochondria

13.5K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
13.5K
Mitochondrial Membranes01:45

Mitochondrial Membranes

11.7K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
11.7K
Microtubule Instability02:17

Microtubule Instability

5.0K
Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated...
5.0K
Destabilization of Microtubules01:45

Destabilization of Microtubules

2.9K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
2.9K
Actin Treadmilling01:18

Actin Treadmilling

7.9K
Actin filaments undergo polymerization and depolymerization from either end. The polymerization and depolymerization rates depend on the cytosolic concentration of free G-actins. The polymerization rate is generally higher at the plus or barbed end, while the depolymerization rate is higher at the minus or pointed end. At a steady state, critical concentration describes the concentration of free G-actin monomers at which the polymerization rate at the plus end is equal to that of the...
7.9K
Adaptability of Cytoskeletal Filaments01:12

Adaptability of Cytoskeletal Filaments

5.7K
The cytoskeleton is a complex dynamic structure performing varied functions based on cellular requirements. The adaptability of the individual filaments in the cytoskeleton determines their ability to perform various functions within the cell. It can undergo rapid reorganization during processes like cell division or remain stable for several hours as in the interphase. The adaptability of these filaments depends on stringent regulatory mechanisms. The microfilament and microtubules of the...
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Does closed suction drainage reduce postoperative hematoma and muscle swelling after total hip arthroplasty? A retrospective comparative study using computed tomography.

Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs·2026
Same author

APOE is a presynaptic protein that accumulates with age and modulates neurotransmitter release.

bioRxiv : the preprint server for biology·2026
Same author

Depth of DNA photodamage in human corneas exposed to 222 nm or 254 nm UV.

Photochemistry and photobiology·2026
Same author

Spontaneous transition of double tachycardia with atrial fusion between atrioventricular reciprocating tachycardia and atrial tachycardia: a case report.

Journal of cardiology cases·2026
Same author

Immune Checkpoint Inhibitor-Induced Diabetes Across National Cancer Institute Trials That Included PD-1 or PD-L1 Agents.

JAMA oncology·2025
Same author

Glutamate indicators with increased sensitivity and tailored deactivation rates.

Nature methods·2025

Related Experiment Video

Updated: May 5, 2026

Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging
05:29

Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging

Published on: January 17, 2025

1.9K

Actin dynamics affect mitochondrial quality control and aging in budding yeast.

Ryo Higuchi1, Jason D Vevea, Theresa C Swayne

  • 1Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, 630 W. 168(th) Street, New York, NY 10032, USA.

Current Biology : CB
|November 26, 2013
PubMed
Summary

Retrograde actin cable flow (RACF) in yeast acts as a filter, ensuring only fit mitochondria are inherited by daughter cells. Enhancing RACF improves mitochondrial health and cellular lifespan.

More Related Videos

Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast
10:41

Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast

Published on: August 20, 2013

12.2K
A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae
10:39

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

6.1K

Related Experiment Videos

Last Updated: May 5, 2026

Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging
05:29

Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging

Published on: January 17, 2025

1.9K
Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast
10:41

Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast

Published on: August 20, 2013

12.2K
A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae
10:39

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

6.1K

Area of Science:

  • Cell Biology
  • Genetics
  • Biochemistry

Background:

  • Actin cables in budding yeast facilitate intracellular transport.
  • Retrograde actin cable flow (RACF) is a conserved cellular process.
  • Mitochondria must overcome RACF for inheritance by daughter cells.

Purpose of the Study:

  • To investigate the role of RACF in mitochondrial inheritance and cellular health.
  • To determine the impact of RACF rates on mitochondrial fitness and lifespan.
  • To elucidate the involvement of the sirtuin SIR2 in RACF and mitochondrial quality control.

Main Methods:

  • Observation of actin cable dynamics and mitochondrial movement in yeast.
  • Manipulation of RACF rates and assessment of mitochondrial fitness.
  • Genetic analysis of SIR2 function in relation to RACF and lifespan.

Main Results:

  • Increased RACF rates enhance the fitness of inherited mitochondria, extending cellular lifespan and healthspan.
  • The sirtuin SIR2 is essential for normal RACF and mitochondrial fitness.
  • Accelerating RACF in sir2Δ cells improves mitochondrial and cellular health, but not replicative lifespan.

Conclusions:

  • RACF acts as a crucial filter for segregating fit from less-fit mitochondria during inheritance.
  • This filtering mechanism by RACF significantly influences cellular lifespan and healthspan.
  • Sir2p plays a vital role in regulating RACF, mitochondrial fitness, and cellular aging.