Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

8.9K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.9K
Production of Pharmaceuticals01:30

Production of Pharmaceuticals

99
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
99
Gram-negative Bacterial Protein Secretion Systems01:17

Gram-negative Bacterial Protein Secretion Systems

1.7K
Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
1.7K
Synthetic Biology02:55

Synthetic Biology

4.4K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
4.4K
Regulation of Bacterial Virulence01:28

Regulation of Bacterial Virulence

80
Pathogenic bacteria employ a range of regulatory mechanisms to modulate the expression of virulence genes in response to environmental and host-derived signals. These mechanisms ensure that virulence factors are expressed only under favorable conditions, thereby optimizing infection and survival strategies.Mechanisms of Virulence RegulationKey regulatory strategies include:Two-Component Systems: These consist of a membrane-bound sensor kinase and a cytoplasmic response regulator. Environmental...
80
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

1.2K
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Soluble multi-epitope protein vaccination leverages antigen availability to drive CD8<sup>+</sup> T cell immunity and tumor control.

Frontiers in immunology·2026
Same author

Tracking spatio-temporal dynamics of early immune responses to an intranasal OMV-based pneumococcal vaccine candidate in mice.

NPJ vaccines·2026
Same author

Verapamil Suppresses the Development of Resistance Against Anti-Tuberculosis Drugs in Mycobacteria.

International journal of molecular sciences·2025
Same author

Overproduction of β-barrel outer membrane proteins in <i>Escherichia coli</i> BL21(DE3) induces hypervesiculation.

Extracellular vesicles and circulating nucleic acids·2025
Same author

Targeting WWPI HECT Domain by Small Inhibitors for Restoring PTEN Tumor Suppressive Role in Glioblastoma Therapy.

Anti-cancer agents in medicinal chemistry·2025
Same author

Impact of expression systems on the transcriptome of <i>Bacillus subtilis</i>: insights for enhanced production of glutaminase PrgA.

Applied and environmental microbiology·2025

Related Experiment Video

Updated: May 5, 2026

A Visual Assay to Monitor T6SS-mediated Bacterial Competition
08:45

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

Published on: March 20, 2013

19.6K

Type V secretion: from biogenesis to biotechnology.

Peter van Ulsen1, Sadeeq ur Rahman1, Wouter S P Jong2

  • 1Section of Molecular Microbiology, Department of Molecular Cell Biology, VU University, 1081 HV Amsterdam, The Netherlands.

Biochimica Et Biophysica Acta
|November 26, 2013
PubMed
Summary

Gram-negative bacteria use Type V secretion systems, including autotransporters and two-partner secretion (TPS) systems, for protein transport across their outer membrane. These systems are crucial for bacterial survival and have biotechnological applications.

Keywords:
AutotransporterAutotransporter secretionBiotechnological applicationProtein foldingSurface displayTwo-partner secretion

More Related Videos

Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells
09:25

Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells

Published on: August 19, 2016

12.3K
Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
12:03

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

Published on: January 22, 2014

33.0K

Related Experiment Videos

Last Updated: May 5, 2026

A Visual Assay to Monitor T6SS-mediated Bacterial Competition
08:45

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

Published on: March 20, 2013

19.6K
Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells
09:25

Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells

Published on: August 19, 2016

12.3K
Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
12:03

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

Published on: January 22, 2014

33.0K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Gram-negative bacteria possess complex protein secretion systems to interact with their environment.
  • Specialized secretion systems, including Type V, are essential for protein translocation across the cell envelope.
  • Type V secretion systems are vital for bacterial virulence and survival, and useful for biotechnology.

Purpose of the Study:

  • To review the biogenesis and applications of two Type V secretion system subtypes: autotransporters and two-partner secretion (TPS) systems.
  • To elucidate the mechanisms of protein translocation across the outer membrane in Gram-negative bacteria.
  • To highlight the role of these systems in outer membrane protein assembly and their biotechnological potential.

Main Methods:

  • Review of existing literature on Type V secretion systems, autotransporters, and TPS systems.
  • Analysis of the roles of the Bam complex and dedicated translocators in protein secretion.
  • Comparison of mechanistic studies and applied research on these secretion pathways.

Main Results:

  • Autotransporters utilize the general outer membrane assembly machinery (Bam complex) for translocation.
  • TPS systems employ a dedicated translocator with similarities to BamA.
  • Studies reveal insights into secretion mechanisms and outer membrane protein biogenesis.

Conclusions:

  • Type V secretion systems are critical for Gram-negative bacterial functions and biotechnological applications.
  • Understanding these systems enhances knowledge of outer membrane protein assembly.
  • Further research into autotransporters and TPS systems holds promise for both basic science and applied fields.