Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

16.3K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
16.3K
Crossing Over01:34

Crossing Over

128.7K
Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process...
128.7K
Crossing Over01:30

Crossing Over

6.4K
Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I,...
6.4K
Polytene Chromosomes02:04

Polytene Chromosomes

9.3K
Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also...
9.3K
Polytene Chromosomes02:04

Polytene Chromosomes

2.8K
2.8K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.7K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microtuberization in potato (Solanum tuberosum L.).

Plant cell reports·2019
Same author

A translocation tester set in pearl millet.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Characterization of nuclear and cytoplasmic information in the progeny of a somatic hybrid between male sterile Nicotiana tabacum and N. glutinosa.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Peroxidase isozyme patterns in primary trisomics of pearl millet.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Development of monosomic alien addition lines and introgression of genes from Oryza australiensis Domin. to cultivated rice O. sativa L.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Intergeneric somatic hybrids of rice [Oryza sativa L. (+) Porteresia coarctata (Roxb.) Tateoka].

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2012

Related Experiment Video

Updated: May 5, 2026

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
06:10

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

6.6K

Multiple chromosomal interchanges in pearl millet.

D S Brar1, J L Minocha

  • 1Department of Genetics, Punjab Agricultural University, Ludhiana, India.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|November 26, 2013
PubMed
Summary
This summary is machine-generated.

Developing multiple chromosome interchanges in pearl millet (Pennisetum typhoides) led to increased sterility. This meiotic instability, caused by translocated chromosomes, hinders gamete selection and establishing homozygous lines.

More Related Videos

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
09:32

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

7.3K
Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
09:45

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

9.3K

Related Experiment Videos

Last Updated: May 5, 2026

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
06:10

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

6.6K
An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
09:32

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

7.3K
Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
09:45

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

9.3K

Area of Science:

  • Plant Genetics
  • Cytology
  • Pearl Millet Breeding

Background:

  • Pearl millet (Pennisetum typhoides) is a vital cereal crop.
  • Understanding chromosomal rearrangements is crucial for crop improvement.
  • Previous studies explored chromosome interchanges in various species.

Purpose of the Study:

  • To investigate the development of multiple interchanges in pearl millet.
  • To assess the meiotic behavior and sterility associated with these interchanges.
  • To evaluate the potential of multiple interchange heterozygotes for breeding applications.

Main Methods:

  • Employing intercrossing and irradiation techniques to induce chromosomal interchanges.
  • Analyzing meiotic configurations in interchange heterozygotes.
  • Quantifying pollen and ovule sterility in developed lines.

Main Results:

  • Successfully created multiple interchanges involving up to the entire chromosome complement in pearl millet.
  • Observed high frequencies (90.9% and 87.8%) of abnormal meiotic cells in heterozygotes with 12 and 14 chromosomes involved in interchanges.
  • Demonstrated a direct correlation between the number of translocated chromosomes and increased pollen/ovule sterility.

Conclusions:

  • Meiotic instability, deficiency-duplication gametes, and unequal chromosome distribution contribute to sterility in multiple interchange heterozygotes.
  • Despite predominant alternate segregation, sterility is a significant barrier to utilizing multiple interchanges for gamete selection and homozygous line development in pearl millet.