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Perivascular macrophages mediate neutrophil recruitment during bacterial skin infection

Arby Abtin1, Rohit Jain1, Andrew J Mitchell1

  • 11] The Centenary Institute, Newtown, New South Wales, Australia. [2].

Nature Immunology
|November 26, 2013
PubMed

Insights

Perivascular macrophages are crucial for neutrophil migration during Staphylococcus aureus skin infections. Staphylococcus aureus uses alpha-hemolysin to kill these macrophages, hindering neutrophil recruitment and aiding immune evasion.

Area of Science:

  • Immunology
  • Microbiology
  • Cell Biology

Background:

  • Neutrophil transendothelial migration is vital for inflammatory responses.
  • The regulation of neutrophil migration into infected tissues is not fully understood.

Purpose of the Study:

  • To investigate the role of perivascular macrophages in neutrophil recruitment to skin infected with Staphylococcus aureus.
  • To elucidate the mechanism by which Staphylococcus aureus affects neutrophil migration.

Main Methods:

  • Multiphoton intravital microscopy was used to visualize neutrophil migration in vivo.
  • Experiments involved infection with Staphylococcus aureus and analysis of perivascular macrophages and neutrophil transmigration.

Main Results:

  • Neutrophils extravasate near perivascular macrophages in inflamed skin.
  • Perivascular macrophages are a significant source of neutrophil chemoattractants.
  • Staphylococcus aureus alpha-hemolysin lyses perivascular macrophages, reducing neutrophil transmigration.

Conclusions:

  • Perivascular macrophages play a critical, previously unrecognized role in neutrophil recruitment to inflamed skin.
  • Staphylococcus aureus employs alpha-hemolysin to eliminate perivascular macrophages as an immune evasion strategy.

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