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Related Experiment Video

Updated: May 5, 2026

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula

Published on: June 9, 2015

Somatic embryogenesis in Zea mays L.

C Lu¹, I K Vasil, P Ozias-Akins

¹Department of Botany, University of Florida, Gainesville, Fla., USA.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik

|November 26, 2013

Summary

Immature Zea mays embryos successfully developed into mature plants through somatic embryogenesis. This research demonstrates a reliable method for plant regeneration from Zea mays (corn) callus cultures.

Area of Science:

Plant Biotechnology
Agricultural Science
Developmental Biology

Background:

Somatic embryogenesis is a key process for plant regeneration.
Efficient regeneration protocols are crucial for crop improvement in Zea mays (corn).

Purpose of the Study:

To establish an efficient somatic embryogenesis and plant regeneration protocol for 12 Zea mays genotypes.
To investigate the potential of scutellar callus for generating embryogenic cell suspensions.

Main Methods:

Immature embryos from 12 Zea mays genotypes were cultured on media with 2,4-dichlorophenoxyacetic acid and sucrose.
Callus proliferation from scutellar cells was induced.
Somatic embryos were developed from callus and regenerated into whole plants.

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Last Updated: May 5, 2026

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula

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Published on: June 9, 2015

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

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Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

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Published on: February 14, 2020

Main Results:

Organized, embryogenic callus formed from scutellar cells.
Well-organized somatic embryos developed and germinated into normal, mature green plants.
An embryogenic cell suspension culture was established for protoplast isolation.

Conclusions:

Somatic embryogenesis provides a viable route for regenerating mature Zea mays plants from immature embryos.
The established protocol is effective across multiple genotypes, offering potential for genetic improvement.
The embryogenic cell suspension culture is a valuable resource for further biotechnological applications, including protoplast research.