Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nuclear Export of mRNA02:31

Nuclear Export of mRNA

7.1K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.1K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

4.7K
4.7K
RNA Splicing01:32

RNA Splicing

53.5K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
53.5K
RNA Splicing01:32

RNA Splicing

15.9K
15.9K
Nuclear Export01:42

Nuclear Export

3.7K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
3.7K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.6K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The fluorescence-activating and absorption-shifting tag (FAST), a versatile protein marker for live plant cell imaging.

The Plant journal : for cell and molecular biology·2026
Same author

2D Multimodal Image Collection for Fluorescence Prediction from Transmitted Light Microscopy.

Scientific data·2026
Same author

An integrative structural biology approach reveals the dynamic organization of the R2SP quaternary chaperone complex.

Nature communications·2026
Same author

Co-translational determination of quaternary structures in chaperone factories.

Nature communications·2026
Same author

A deep learning approach for time-consistent cell cycle phase prediction from microscopy data.

PLoS computational biology·2025
Same author

Chemical inhibition of exon junction complex assembly impairs mRNA localization and neural stem cells ciliogenesis.

Nucleic acids research·2025

Related Experiment Video

Updated: May 5, 2026

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
10:40

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

Published on: December 28, 2016

7.3K

The human cap-binding complex is functionally connected to the nuclear RNA exosome.

Peter Refsing Andersen1, Michal Domanski, Maiken S Kristiansen

  • 11] Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark. [2].

Nature Structural & Molecular Biology
|November 26, 2013
PubMed
Summary

The cap-binding complex (CBC) links RNA processing factors to the nuclear exosome targeting (NEXT) complex. This connection aids in suppressing faulty RNA transcripts by promoting transcription termination.

More Related Videos

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

8.5K
Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

9.2K

Related Experiment Videos

Last Updated: May 5, 2026

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
10:40

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

Published on: December 28, 2016

7.3K
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

8.5K
Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

9.2K

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • RNA Metabolism

Background:

  • The eukaryotic RNA exosome degrades RNA molecules, ensuring proper gene expression.
  • Accessory factors regulate the RNA exosome, but how it targets specific RNA molecules is unclear.
  • Understanding exosome recruitment is crucial for controlling RNA processing and quality.

Purpose of the Study:

  • To elucidate the mechanism of RNA exosome recruitment to its ribonucleoprotein (RNP) targets.
  • To identify the physical and functional links between the cap-binding complex (CBC) and the nuclear exosome.
  • To investigate the role of the CBC-ARS2 and CBC-NEXT complexes in RNA processing.

Main Methods:

  • RNA immunoprecipitation assays using components of the CBC-NEXT (CBCN) complex.
  • Combinatorial depletion analysis of CBCN and exosome factors.
  • Investigating the impact of CBCN on transcriptional termination and RNA degradation.

Main Results:

  • A physical link was established between the human cap-binding complex (CBC) and the nuclear exosome targeting (NEXT) complex via ARS2 and ZC3H18 proteins, forming CBC-NEXT (CBCN).
  • CBCN complex formation was shown to be functionally relevant for targeting RNA molecules.
  • The CBC-ARS2 (CBCA) complex was found to suppress read-through RNA products by promoting transcriptional termination.

Conclusions:

  • The RNP 5' cap acts as a crucial link between transcription termination and exosomal RNA degradation through the CBC-NEXT complex.
  • This mechanism highlights a novel pathway for RNA quality control and gene expression regulation.
  • The findings provide new insights into the coordinated processes of transcription, RNA processing, and degradation.