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Updated: May 5, 2026

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Published on: February 5, 2015
Expression of Anabaena ferredoxin genes in Escherichia coli
1Department of Molecular Genetics and Cell Biology, University of Chicago, 60637, Chicago, IL, USA.
Abstract:
The genes for ferredoxin from heterocysts (fdx H) and vegetative cells (pet F) of Anabaena sp. strain 7120 were subcloned into plasmid pUC 18/19. Both genes were expressed in Escherichia coli at high levels (≈10% of total protein). Pet F could be expressed from its own promoter. The ferredoxins were correctly assembled to the holoprotein. Heterocyst ferredoxin was purified from E. coli extracts on a large scale. Its biochemical and biophysical properties were identical to those of the authentic ferredoxin, isolated from Anabaena heterocysts.
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