Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure and function of cytochrome-c oxidase.

M Denis

    Biochimie
    |March 1, 1986
    PubMed
    Summary

    This review covers cytochrome-c oxidase structure and function, detailing its varied subunit composition, metal centers, and Y-shaped mitochondrial structure. It highlights enzyme conformers, active forms, and its role as a proton pump.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    <i>Salmonella</i> in pigs slaughtered in Ecuador: prevalence, serotypes, genotypes and antibiotic resistance.

    Veterinary and animal science·2025
    Same author

    Neurofeedback and epilepsy: Renaissance of an old self-regulation method?

    Revue neurologique·2024
    Same author

    Toward resilient food systems after COVID-19.

    Current research in environmental sustainability·2022
    Same author

    From hypotonic maintenance fluid to severe hyponatremia: a case report.

    Journal of medical case reports·2021
    Same author

    Daily multidisciplinary COVID-19 meeting: Experiences from a French university hospital.

    Respiratory medicine and research·2021
    Same author

    Caffeine treatment for bronchiolitis-related apnea in the pediatric intensive care unit.

    Archives de pediatrie : organe officiel de la Societe francaise de pediatrie·2019

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Bioenergetics

    Background:

    • Cytochrome-c oxidase (CcO) is a crucial enzyme in cellular respiration.
    • Its structure and function are complex, varying between species and cellular compartments.

    Purpose of the Study:

    • To review recent advancements in the structure and function of cytochrome-c oxidase.
    • To consolidate knowledge on enzyme composition, topology, metal centers, and catalytic mechanisms.

    Main Methods:

    • Literature review of protein sequencing, gene sequencing, and spectrometric techniques.
    • Analysis of data from reconstitution experiments and kinetic studies.
    • Investigation of enzyme conformers and intermediate reaction states.

    Main Results:

    • CcO subunit composition ranges from 5-13 in eukaryotes to 1-3 in prokaryotes.
    • Metal centers (Fea, CuA) are located in subunits I and II; enzyme structure is Y-shaped in mitochondria.
    • Characterization of 'pulsed,' 'resting,' and 'oxygenated' conformers, and evidence for proton pumping involving subunit III.

    Conclusions:

    • The review synthesizes current understanding of CcO structure, function, and proton pumping mechanism.
    • Further research is needed to elucidate the precise molecular mechanism of proton pumping.
    • Understanding CcO's active forms and lipid roles remains critical.

    Related Experiment Videos