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Intestinal iron absorption in chickens : I. Experimental conditions
M T Marti1, M P Saiz, M T Mitjavila
1Department of Animal Physiology, Faculty of Biology, University of Barcelona, Barcelona, Spain.
Biological Trace Element Research
|November 26, 2013
Summary
Chicken intestinal iron absorption is linear with concentration up to a saturation point. Plasma binding capacity may regulate iron uptake, with 30-60 minute perfusion periods being optimal for study.
Area of Science:
- Animal Physiology
- Gastrointestinal Physiology
- Nutritional Biochemistry
Background:
- Understanding intestinal iron absorption is crucial for poultry nutrition and health.
- Previous studies have focused on mammalian models, necessitating specific research in avian species.
- The regulatory mechanisms of iron uptake in chickens remain incompletely understood.
Purpose of the Study:
- To investigate the kinetics of intestinal iron absorption in chickens in vivo.
- To determine the relationship between iron concentration, absorption time, and saturation.
- To explore potential regulatory factors influencing iron absorption in the avian gut.
Main Methods:
- Utilized an in vivo closed-circuit intestinal perfusion technique in chickens.
- Assessed iron absorption at varying iron concentrations (up to 776 μg Fe/20 mL) and time intervals (15, 30, 60, 120 min).
- Analyzed the linearity and saturation kinetics of iron absorption.
Main Results:
- Intestinal iron absorption demonstrated a linear relationship with iron concentration up to 776 μg Fe/20 mL, beyond which saturation occurred.
- Absorption rate showed a linear correlation with time for a given iron concentration (14 μg Fe/20 mL).
- Mucosal epithelial cells appeared less limiting in chickens compared to rats, suggesting a significant role for plasma binding capacity in iron regulation.
Conclusions:
- Chicken intestinal iron absorption follows Michaelis-Menten-like kinetics, with saturation occurring at higher concentrations.
- Plasma binding capacity is likely a key regulator of iron absorption in chickens.
- Perfusion periods of 30-60 minutes with low iron concentrations (14 μg Fe/20 mL) are suitable for studying avian intestinal iron absorption without saturation effects.
