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Determination of β-Endorphin and Fragments Thereof in Human Plasma Using High-Performance Liquid Chromatography and a
1Rudolf Buchheim-Institut für Pharmakologie der Justus Liebig-Universität, Frankfurter Str. 107, D-6300, Gieβen, FRG.
Abstract:
A method for the determination of β-endorphin and β-endorphin fragments in human plasma was developed. β-Endorphin-related peptides were extracted from plasma using octadecasilyl-silica cartridges. Extracts were subjected to re versed-phase high-performance liquid chromatography (HPLC). Extracts as well as HPLC column eluates were assayed using a multiple radioimmunoassay system; several antibodies directed against various distinct regions of the β-endorphin molecule were employed. Using this method, evidence for the presence of multiple β-endorphin fragments in the plasma of healthy young volunteers (under normal conditions) was obtained.
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