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Free nucleotides in bromodeoxyuridine-treated neuroblastoma cells

M Wintzerith¹, A Dierich, J Ciesielski-Treska

¹Centre de Neurochimie du CNRS, 11, Rue Humann, 67085, Strasbourg Cedex, France.

Neurochemical Research

|November 26, 2013

Abstract:

Free nucleotides in bromodeoxyuridine-treated neuroblastoma cells were investigated. The separation and purification of nucleotides were carried out by anion exchange and paper chromatography. After bromodeoxyuridine treatment, the distribution of free nucleotides with the same base, when referred to total nucleotides, shifted toward a distribution pattern closer to that of mature neuronal cells. There was an enhancement of adenylic nucleotides and a decrease of uridylic and cytidylic nucleotides. The guanylic nucleotides displayed no changes. This nucleotide distribution is in agreement with other biochemical data from differentiated neuroblastoma cells.

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