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Updated: May 5, 2026
![Radiosynthesis of 1-2-[18F]Fluoroethyl-L-Tryptophan using a One-pot, Two-step Protocol](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F63025.jpg&w=3840&q=50)
Radiosynthesis of 1-2-[18F]Fluoroethyl-L-Tryptophan using a One-pot, Two-step Protocol
Published on: September 21, 2021
5-Hydroxytryptophan: A method for monitoring human plasma content during clinical administration
P Angwin1, P Berger, V Zarcone
1Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, 94305, Stanford, California.
Abstract:
A method is described for the assay of 5-hydroxytryptophan (5-HTP) in human blood plasma following administration of the compound. The procedure involves extracting the amino acid into butanol, returning the 5-HTP to an aqueous phase, and separating it from other interfering 5-hydroxyindoles on a column of Dowex 50W-X4, a strong cation-exchange resin. The concentration of 5-HTP in the buffer eluate is determined spectrofluorometrically in 3 N HCl; 25 ng/ml plasma can be detected. Using this method, it was possible to determine the time-course of plasma 5-HTP concentrations during the 24-h period after a single 300-mg dose. 5-HTP is being utilized in clinical trials for several psychiatric and neurological disorders.
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