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Related Concept Videos

Enzyme-linked Receptors01:00

Enzyme-linked Receptors

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Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
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Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

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Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
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Related Experiment Video

Updated: May 5, 2026

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate
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Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate

Published on: September 18, 2013

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Pyruvate kinase binding to particles in brain.

H R Knull1

  • 1Department of Oral Biology, University of Manitoba, R3E OW3, Winnipeg, Manitoba, Canada.

Neurochemical Research
|November 26, 2013
PubMed
Summary

Pyruvate kinase interacts electrostatically with rat brain membranes. Enzyme release from membranes and developmental changes were analyzed, showing synchronous increases in both forms.

Area of Science:

  • Biochemistry
  • Neuroscience
  • Enzymology

Background:

  • Pyruvate kinase is a key glycolytic enzyme.
  • Its localization in brain tissue is not fully understood.
  • Membrane-bound enzymes play crucial roles in cellular processes.

Purpose of the Study:

  • To investigate the nature of pyruvate kinase interaction with rat brain membranes.
  • To characterize the solubilization of membrane-bound pyruvate kinase.
  • To examine the developmental changes in pyruvate kinase forms.

Main Methods:

  • Studying enzyme-membrane interactions using electrostatic properties.
  • Employing ionic compounds and pH variations for enzyme solubilization.
  • Analyzing developmental patterns of particulate and soluble pyruvate kinase.

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Main Results:

  • Pyruvate kinase interaction with brain membranes is electrostatic.
  • High pH and ionic compounds effectively solubilize the enzyme.
  • Particulate and soluble pyruvate kinase levels increase together during development.

Conclusions:

  • The binding of pyruvate kinase to brain membranes is primarily ionic.
  • Solubilization is dependent on ionic strength and pH.
  • Brain pyruvate kinase exhibits coordinated developmental expression of its forms.