Related Experiment Video
Updated: May 5, 2026

Co-localization of Cell Lineage Markers and the Tomato Signal
Published on: December 28, 2016
Organisation and expression of a wound/ripening-related small multigene family from tomato
M J Holdsworth1, W Schuch, D Grierson
1Department of Physiology and Environmental Science, University of Nottingham, School of Agriculture, Sutton Bonington, LE12 5RD, Loughborough, UK.
Abstract:
cDNA clones derived from a ripe tomato fruit cDNA library were used to investigate changes in the abundance of specific mRNAs in ripening fruit and wounded leaves. mRNAs related to one cDNA clone (pTOM 13) were expressed in both situations. This clone was used to identify homologous sequences in a tomato genomic library. Three groups of related clones that hybridised to the pTOM 13 cDNA insert were identified and subcloned into plasmid vectors. Genomic Southern analysis of tomato DNA using gene-specific DNA fragments isolated from the subcloned DNAs indicated that all pTOM 13 closely related genes had been isolated. RNA dot blot analysis with these DNA fragments as probes indicated differential expression of this small multigene family in leaves and fruit.
More Related Videos
09:05Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
Published on: March 11, 2020
15:25Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects
Published on: March 16, 2010
Related Concept Videos
Fruit Development, Structure, and Function
Regulation of Expression at Multiple Steps
Canonical Wnt Signaling Pathway
Canonical Wnt Signaling Pathway
Gene Regulation During Sporulation
Regulation of Expression Occurs at Multiple Steps
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...