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Updated: May 5, 2026

Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates
Published on: July 2, 2018
Bacteroid-encoded proteins are secreted into the peribacteroid space by Rhizobium leguminosarum
P Katinakis1, R M Lankhorst, J Louwerse
1Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC, Wageningen, Netherlands.
Abstract:
Bacteroids of Rhizobium leguminosarum in root nodules of Pisum sativum are enclosed by a plant-derived peribacteriod membrane (PBM). The contents of the interstitial peribacteroid space (PBS) between bacteroid membrane and PBM were isolated by a controlled osmotic shock of PBM-enclosed bacteroids and analysed by two-dimensional gel electrophoresis. Silver staining revealed approximately 40 PBS polypeptides. Ex planta (35)S-methionine labeling of PBM-enclosed bacteroids revealed that about 90% of the PBS proteins are synthesized by the bacteroid. Approximately 30% of the PBS polypeptides are common between the PBS and the periplasmic space of free-living bacteria; one (38kDa) PBS protein is also excreted by free-living bacteria in the bacterial culture medium. At least four bacteroid-encoded PBS polypeptides were clearly identified as symbiosis-specific.
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