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MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
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Computational prediction of microRNA function and activity.

Hasan Oğul1

  • 1Department of Computer Engineering, Baskent University, Ankara, Turkey.

Methods in Molecular Biology (Clifton, N.J.)
|November 26, 2013
PubMed
Summary

Understanding microRNA (miRNA) functions is crucial for cell biology. This chapter details methods for predicting miRNA activity and analyzing their regulatory roles in gene expression.

Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Genetics

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression.
  • Understanding miRNA function is vital for deciphering cellular mechanisms and gene behavior.
  • System-level roles of miRNAs remain an active area of research.

Purpose of the Study:

  • To outline methodologies for predicting miRNA function and activity.
  • To introduce computational approaches for miRNA data analysis.
  • To explore information flow mediated by miRNAs.

Main Methods:

  • Review of existing methodologies for miRNA function prediction.
  • Introduction to computational tools for sequence and experimental data analysis.
  • Framework for analyzing miRNA-mediated information flow.

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Main Results:

  • Provides a comprehensive overview of miRNA function inference techniques.
  • Highlights computational methods for investigating miRNA roles.
  • Establishes a basis for understanding miRNA regulatory networks.

Conclusions:

  • Accurate inference of miRNA function and activity is essential for systems biology.
  • Computational approaches are powerful tools for dissecting miRNA-mediated gene regulation.
  • This chapter serves as a guide to analyzing miRNA roles in cellular processes.