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Updated: May 5, 2026

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
Published on: July 28, 2016
Possible involvement of nodule superoxide dismutase and catalase in leghemoglobin protection
A Puppo1, L Dimitrijevic, J Rigaud
1Faculté des Sciences et des Techniques, Laboratoire de Biologie Végétale, Parc Valrose, F-06034, Nice Cédex, France.
Abstract:
Superoxide anion is able to oxidize oxyleghemoglobin prepared from soybean nodules. Furthermore, ferrileghemoglobin is oxidized to leghemoglobin (IV) by hydrogen peroxide and this irreversible reaction leads to a complete inactivation of the hemoprotein. In scavenging O 2 (-) and H2O2, superoxide dismutase (EC 1.15.1.1) and catalase (EC 1.11.1.6) are able to limit these oxidations. The occurrence of these enzymes within soybean nodules and their main characteristics are reported here. A general scheme taking into account their roles in leghemoglobin protection in vivo is proposed.
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