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Updated: May 5, 2026

Determination of the Glycogen Content in Cyanobacteria
Published on: July 17, 2017
Rice cytosolic glyceraldehyde 3-phosphate dehydrogenase contains two subunits differentially regulated by
1Station de Physiologie Végétale, I.N.R.A., 33140, Pont-de-la Maye, France.
Abstract:
Rice cytosolic glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is composed of two subunits of different molecular weights. Cytosolic GAPDH activity and protein both decreased immediately after transfer of 48-h rice seedlings to anaerobic conditions. Subsequent increase in activity and protein was accompanied by a change in isoenzyme profile and was preceded by an increase in steady-state messenger levels. One and two-dimensional electrophoretic analyses of in vivo and in vitro labeled GAPDH suggested that the change in isoenzyme profile under anaerobic conditions is due to preferential synthesis of one of the two GAPDH subunits caused by a specific increase in its mRNA.
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