Related Experiment Video
Updated: May 5, 2026

Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants
Published on: November 1, 2016
Preparation of a tRNA-dependent wheat germ protein-synthesizing system
H Pfitzinger1, J H Weil, D T Pillay
1Institut de Biologie Moléculaire des Plantes, Université Louis Pasteur, 12 rue du Général Zimmer, 67000, Strasbourg, France.
Abstract:
A method is described for the preparation of a tRNA-dependent wheat germ protein-synthesizing system. This system can be supplied with exogenous tRNAs of eukaryotic or prokaryotic origin. In order to obtain maximal aminoacylation of the added tRNAs, the translation assays can be supplemented with homologous aminoacyl-tRNA synthetase preparations. Such a tRNA-dependent wheat germ protein-synthesizing system, which is easy to prepare, can be used not only to translate plant cytoplasmic mRNAs in the presence of added cytoplasmic tRNAs, but also to determine the translation activity of exogenous tRNAs from various sources in the presence of either natural or in vitro synthesized mRNAs.
More Related Videos
09:31PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
Published on: September 26, 2020
07:43Author Spotlight: Optimizing CFPS Systems for Synthetic Cell Construction
Published on: April 19, 2024