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Peribacteroid membrane nodulin gene induction by Bradyrhizobium japonicum mutants

R B Mellor1, C Garbers, D Werner

  • 1Botanisches Institut, Fachbereich Biologie der Philipps-Universität Marburg, D-3550, Marburg, Federal Republic of Germany.

Plant Molecular Biology
|November 26, 2013
PubMed

Insights

Soybean root mRNA analysis reveals nodule-specific proteins. Bradyrhizobium japonicum infection influences the production of these nodulins, suggesting microbial signaling in peribacteroid membrane formation.

Area of Science:

  • Plant-microbe interactions
  • Molecular biology
  • Soybean nodulation

Background:

  • Peribacteroid membranes are crucial for symbiotic nitrogen fixation in legumes.
  • Nodules contain specific proteins (nodulins) synthesized by the plant in response to rhizobial infection.

Purpose of the Study:

  • To identify nodule-specific polypeptides synthesized by Glycine max (soybean).
  • To investigate the influence of Bradyrhizobium japonicum infection on nodulin expression and peribacteroid membrane formation.

Main Methods:

  • Translation of Glycine max root mRNA.
  • Immunoprecipitation using antiserum against peribacteroid membrane preparations.
  • Analysis of nodule-specific polypeptides under different B. japonicum infection conditions.

Main Results:

  • Seventeen nodulins were identified from effective nodules.
  • Fewer nodulins were detected in nodules infected with B. japonicum strains causing defective or empty peribacteroid membranes.
  • The expression of at least 4 nodulins correlated with peribacteroid membrane integrity.

Conclusions:

  • The microsymbiont (Bradyrhizobium japonicum) likely produces multiple signals that regulate plant-synthesized nodulins.
  • These signals are essential for proper peribacteroid membrane formation and symbiotic function.

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