Related Experiment Video
Updated: May 5, 2026
Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage CoTrAC
Published on: March 15, 2013
[Bioinformatics analysis and identification of transcriptional regulation of human UCA1 gene]
1Center for Translational Medicine, First Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi'an 710061, China.E-mail: xuemei1984-@163.com.
Objective:
To analyze the transcription factor binding sites and methylation in the promoter region of UCA1 gene.
Methods:
UCA1 gene promoter was analyzed using CpG software to predict the CpG island. MatrixCatch and TFSEARCH were used to predict the potential transcription factor binding sites in UCA1 gene core promoter. ChIP assay was used to identify the transcription factors binding to UCA1 gene core promoter.
Results:
UCA1 gene promoter contained no CpG island and was therefore a typical tissue-specific gene. There were 4 transcription factors associated with human cancers in UCA1 gene core promoter, but only one of them interacted with UCA1 gene core promoter.
Conclusion:
There is no CpG island in UCA1 gene promoter region, and the transcription factor c-Myb can specifically bind to the core promoter.
Related Concept Videos
01:58Cell Specific Gene Expression
02:33Combinatorial Gene Control
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
02:02Cis-regulatory Sequences
01:30General Transcription Factors