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Related Experiment Videos

Interferon induces proliferation in leukemic and normal B-cell subsets.

K H Robért, S Einhorn, L Ostlund

    Hematological Oncology
    |April 1, 1986
    PubMed
    Summary

    Interferon (IFN) can stimulate proliferation in certain B lymphocyte subsets from patients with chronic lymphocytic leukemia (CLL) and healthy donors. These IFN-responsive B-cell subsets are more abundant in spleen tissue than peripheral blood.

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    Area of Science:

    • Immunology
    • Hematology
    • Cell Biology

    Background:

    • Interferon (IFN) is known to modulate immune responses.
    • Chronic lymphocytic leukemia (CLL) is characterized by an accumulation of malignant B lymphocytes.
    • The role of IFN in B-cell proliferation and differentiation in CLL is not fully understood.

    Purpose of the Study:

    • To investigate the in vitro proliferative response of B lymphocyte subsets to interferon (IFN) stimulation.
    • To compare IFN-induced proliferation in B cells from CLL patients, spleen tissue, and healthy donors.
    • To determine if T-cells are required for IFN-induced B-cell proliferation.

    Main Methods:

    • Cell cultures from peripheral blood of CLL patients and healthy donors.
    • Cell cultures from spleen tissue of necro-kidney transplants.

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  • 3H-thymidine incorporation assay to measure DNA synthesis and proliferation following IFN stimulation.

    • Main Results:

    • IFN induced a proliferative response in some normal and leukemic B lymphocyte subsets.
    • These IFN-induced proliferative responses were independent of T-cells.
    • IFN-responsive B-cell subsets were found in higher proportions in spleen tissue compared to peripheral blood.
    • These subsets also constituted a portion of the leukemic lymphocyte pool in some CLL patients.

    Conclusions:

    • Specific B-cell subsets capable of proliferation in response to IFN are present in spleen and peripheral blood.
    • These subsets are more prevalent in spleen tissue and can be part of the leukemic B-cell population in CLL.
    • The heterogeneous distribution and functional characteristics of B-cell subsets may influence the efficacy of IFN therapy in B-cell malignancies.