Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antibiotic-Free Hypothermic Storage of Boar Semen at 5 °C with Next-Day On-Farm Cooling: Fertility and Biosafety Under Field Conditions.

Antibiotics (Basel, Switzerland)·2026
Same author

[Antibiotic-free preservation of boar semen at 5°C: an innovation in pig breeding].

Tierarztliche Praxis. Ausgabe G, Grosstiere/Nutztiere·2026
Same author

Chilling Does Not Affect the Functionality of Intracellular Calcium Stores in Viable Boar Sperm During Liquid Preservation.

International journal of molecular sciences·2026
Same author

Pre-cooling of boar semen before transport in a hot environment enhances biosafety.

Frontiers in microbiology·2025
Same author

Boar Semen Assessment for the Production of Artificial Insemination Doses.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Photodynamic Inactivation of Bacteria in Boar Semen with Blue LED Light.

Microorganisms·2025
Same journal

Telomere regulation of mitochondria in porcine parthenogenetic embryos.

Theriogenology·2026
Same journal

Interaction of ARA54 with androgen receptor in mediating testosterone-dependent regulation of caput epididymal GPX5 expression in mice.

Theriogenology·2026
Same journal

Associations of sow characteristics, boar semen traits, and seminal plasma metabolomics with fertility outcomes following artificial insemination.

Theriogenology·2026
Same journal

Association of 17β-HSD3 with steroidogenesis-related gene expression and primordial germ cell development in ducks.

Theriogenology·2026
Same journal

Farrerol improves the maturation quality of porcine oocytes derived from small follicles associated with RAD51-related DNA repair responses.

Theriogenology·2026
Same journal

Environmentally relevant polycyclic aromatic hydrocarbon mixtures disrupt ovarian endocrine function in the domestic hen (Gallus gallus domesticus).

Theriogenology·2026
See all related articles

Related Experiment Video

Updated: May 5, 2026

Fish Sperm Assessment Using Software and Cooling Devices
07:57

Fish Sperm Assessment Using Software and Cooling Devices

Published on: July 28, 2018

8.7K

Computer-assisted sperm analysis (CASA): capabilities and potential developments.

Rupert P Amann1, Dagmar Waberski

  • 1Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, Colorado, USA.

Theriogenology
|November 27, 2013
PubMed
Summary
This summary is machine-generated.

Computer-assisted sperm analysis (CASA) systems have advanced significantly but basic principles remain. While current CASA technology aids quality assurance and research, it cannot predict fertility. Future systems need improved software and portability for field use.

Keywords:
CASAQuality assuranceSperm analysis

More Related Videos

Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka Oryzias latipes
06:49

Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka Oryzias latipes

Published on: October 6, 2022

4.2K
Author Spotlight: Developing Efficient Cryopreservation and Biobanking Technologies for Global Reef Restoration
05:25

Author Spotlight: Developing Efficient Cryopreservation and Biobanking Technologies for Global Reef Restoration

Published on: June 7, 2024

1.5K

Related Experiment Videos

Last Updated: May 5, 2026

Fish Sperm Assessment Using Software and Cooling Devices
07:57

Fish Sperm Assessment Using Software and Cooling Devices

Published on: July 28, 2018

8.7K
Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka Oryzias latipes
06:49

Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka Oryzias latipes

Published on: October 6, 2022

4.2K
Author Spotlight: Developing Efficient Cryopreservation and Biobanking Technologies for Global Reef Restoration
05:25

Author Spotlight: Developing Efficient Cryopreservation and Biobanking Technologies for Global Reef Restoration

Published on: June 7, 2024

1.5K

Area of Science:

  • Veterinary Reproduction and Andrology
  • Sperm Biology and Analysis

Background:

  • Computer-assisted sperm analysis (CASA) systems have evolved over 40 years with technological advancements.
  • Despite evolution, core concepts for sperm identification and motion pattern analysis remain largely unchanged.
  • CASA systems are widely adopted in major spermatology and semen processing facilities, with varying degrees of reliance.

Observation:

  • Modern CASA systems rapidly capture and analyze images of thousands of sperm from multiple fields.
  • Current systems can assess sperm motion and, in some cases, morphology concurrently.
  • Each marketed CASA system exhibits unique characteristics and capabilities.

Findings:

  • CASA technology, while valuable for quality assurance and research, cannot accurately predict the fertility of a semen sample.
  • Accuracy and precision of CASA output are influenced by numerous factors including species, extender, hardware, software, and operator settings.
  • Semen production facilities may not require significantly different CASA systems, whereas research labs would benefit from systems capable of deep chamber imaging.

Implications:

  • Future CASA software should allow for the identification of sperm subpopulations based on specific attributes, moving beyond population averages.
  • There is a need for portable, field-use CASA systems for on-site analysis of sperm motion and morphology, particularly for human andrology outside urban settings.
  • Advancements in imaging and data processing hardware suggest the feasibility of developing such portable systems.