Extraction, purification and characterization of polysaccharides from Hawk tea
Xuejing Jia1, Chunbang Ding, Shu Yuan
1College of Life and Basic Sciences, Sichuan Agricultural University, Ya'an 625014, China.
Carbohydrate Polymers
|November 27, 2013
Summary
Polysaccharides were extracted and purified from Hawk mature leaf tea. The purified fractions demonstrated significant antioxidant activities, supporting their use in traditional herbal tea applications.
Area of Science:
- Phytochemistry
- Food Science
- Biochemistry
Background:
- Hawk mature leaf tea is a traditional herbal beverage.
- Polysaccharides are known for their diverse biological activities.
- Understanding the composition and function of tea polysaccharides is crucial.
Purpose of the Study:
- To extract, purify, and characterize polysaccharides from Hawk mature leaf tea.
- To determine the optimal extraction conditions for these polysaccharides.
- To evaluate the in vitro antioxidant activities of the purified fractions.
Main Methods:
- Box-Behnken design for optimizing extraction parameters (temperature, time, water-to-solid ratio).
- DEAE-52 chromatography for polysaccharide purification.
- Analysis of sugar composition and molecular weight.
- In vitro antioxidant assays: DPPH radical scavenging and FRAP.
Main Results:
- Optimal extraction achieved at 88.9 °C, 128.2 min, and 11.4 mL/g water-to-solid ratio.
- Two purified fractions, HMP-1 and HMP-2, were obtained with molecular weights of 133 and 100 kDa.
- Fractions composed primarily of arabinose, galactose, glucose, and mannose.
- HMP-1 exhibited strong DPPH radical scavenging and FRAP activities.
Conclusions:
- Hawk mature leaf tea is a viable source of bioactive polysaccharides.
- The purified polysaccharides possess significant antioxidant properties.
- These findings support the traditional use and potential therapeutic applications of Hawk mature leaf tea.
Keywords:
Antioxidant activityLitsea coreana var. lanuginosePolysaccharideResponse surface methodology
