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Updated: May 5, 2026
Ferric Chloride-induced Canine Carotid Artery Thrombosis: A Large Animal Model of Vascular Injury
Published on: September 7, 2018
Evaluation and modification of the overall hemostasis potential assay for use with canine plasma
Anna L Dengate1, Marie-Christine Morel-Kopp, Julia A Beatty
1Northern Blood Research Centre, Kolling Institute of Medical Research, University of Sydney, St Leonards, NSW 2065, Australia., Faculty of Veterinary Science, University of Sydney, Camperdown, NSW 2006, Australia., North Shore Veterinary Specialist Centre, 64 Atchison St, Crows Nest, NSW 2065, Australia.
Objective:
To optimize the overall hemostasis potential (OHP) assay for use with canine platelet-poor plasma and determine reference intervals in healthy dogs.
Animals:
40 healthy dogs.
Procedures:
Blood was collected from the dogs into citrated tubes, and platlet-poor plasma was obtained. The OHP assay and standard coagulation assays (prothrombin time, activated partial thromboplastin time, and fibrinogen concentration) were performed for each sample. The OHP assay outputs were tested for correlations with results of the standard coagulation assays, age, and sex.
Results:
Modifications to the published methodology for the OHP assay were required for use with canine plasma, with less coagulation activator (thrombin) and more fibrinolysis activator (tissue plasminogen activator) than used with human plasma. Male dogs had a higher OHP than did females. High fibrinogen concentrations were associated with increases in maximum optical density, OHP, and overall coagulation potential, and reduced prothrombin time was associated with increases in maximum optical density, overall coagulation potential, OHP, and maximum slope.
Conclusions And Clinical Relevance:
Results supported the use of the OHP assay as an accessible, cost-effective global coagulation assay. Further research is required to determine its clinical application as an alternative to thromboelastography or thrombin generation assays.
