Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

9.8K
9.8K
MicroRNAs01:22

MicroRNAs

3.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
MicroRNAs01:22

MicroRNAs

21.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.2K
DNA Microarrays02:34

DNA Microarrays

16.9K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
16.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

De novo and inherited dominant variants in U4 and U6 snRNA genes cause retinitis pigmentosa.

Nature genetics·2026
Same author

miRScore: A rapid and precise microRNA validation tool.

PLoS computational biology·2025
Same author

Publisher Correction: Analysis of R-loop forming regions identifies RNU2-2 and RNU5B-1 as neurodevelopmental disorder genes.

Nature genetics·2025
Same author

Analysis of R-loop forming regions identifies RNU2-2 and RNU5B-1 as neurodevelopmental disorder genes.

Nature genetics·2025
Same author

High-throughput microRNA sequencing in the developing branchial arches suggests miR-92b-3p regulation of a cardiovascular gene network.

Frontiers in genetics·2025
Same author

An optimised faecal microRNA sequencing pipeline reveals fibrosis in Trichuris muris infection.

Nature communications·2025
Same journal

Correction to 'New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress'.

Nucleic acids research·2026
Same journal

VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

Nucleic acids research·2026
Same journal

Accessibility of telomeric overhangs to stabilizing small-molecule ligands.

Nucleic acids research·2026
Same journal

Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

Nucleic acids research·2026
Same journal

Genome-wide mapping of DNA G-quadruplexes in Trypanosoma brucei chromatin reveals enrichment in coding regions and transcription start sites.

Nucleic acids research·2026
Same journal

Correction to 'The Gene Ontology knowledgebase in 2026'.

Nucleic acids research·2026
See all related articles

Related Experiment Video

Updated: May 5, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

2.2K

miRBase: annotating high confidence microRNAs using deep sequencing data.

Ana Kozomara1, Sam Griffiths-Jones

  • 1Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, UK.

Nucleic Acids Research
|November 27, 2013
PubMed
Summary
This summary is machine-generated.

The miRBase database, a key microRNA repository, has been updated to ensure data quality. A new high-confidence subset of microRNA entries, based on deep sequencing data, is now available to researchers.

More Related Videos

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
06:34

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

7.7K
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

560

Related Experiment Videos

Last Updated: May 5, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

2.2K
A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
06:34

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

7.7K
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

560

Area of Science:

  • Bioinformatics
  • Genomics
  • Molecular Biology

Background:

  • The miRBase database is the primary repository for microRNA sequences.
  • The discovery of novel microRNAs is rapidly increasing, driven by deep sequencing technologies.
  • Maintaining the quality and accuracy of microRNA annotations is a significant challenge due to the volume of new data and varied annotation methods.

Purpose of the Study:

  • To describe recent developments in the miRBase database aimed at improving data quality and user contribution.
  • To introduce a high-confidence subset of microRNA entries.
  • To enhance community engagement and data sharing for microRNA research.

Main Methods:

  • Collation and analysis of small RNA deep sequencing data sets.
  • Development of criteria to assign confidence levels to microRNA entries based on mapped read patterns.
  • Integration of microRNA-specific Wikipedia pages into the miRBase website.

Main Results:

  • The miRBase database (v20, June 2013) contains 24,521 microRNA loci from 206 species, yielding 30,424 mature microRNA products.
  • A high-confidence subset of microRNA entries, validated by deep sequencing data, is now available.
  • The miRBase website now features embedded Wikipedia pages to facilitate community contributions.

Conclusions:

  • The update addresses the challenge of maintaining data quality in a rapidly expanding microRNA sequence repository.
  • The high-confidence subset provides researchers with a curated and reliable dataset.
  • Enhanced community interaction through Wikipedia integration aims to enrich microRNA functional and textual information.