Plantlet regeneration from mesophyll protoplasts of Digitalis lanata Ehrh

Protoplasts were isolated from the mesophyll of Digitalis lanata enzymatically and cultured in a liquid regeneration medium (D2a). Protoplast division occurred at a rate of approximately 30%. Mature cell colonies were transferred onto agar medium (D2b)where they developed into cell clusters with a diameter of about 4-5 mm. After transfer onto MS medium, these calli differentiated leaves and shoots which could be rooted on MS medium containing a low hormone concentration.