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Binding of(51)Cr to human erythrocytes.
1Department of Microbiology, Dental Faculty, University of Oslo, Blindern, Oslo 3, Norway.
Biological Trace Element Research
|November 27, 2013
Summary
Radioactive chromium in human red blood cells exists in two forms, bound to macromolecules or as a low molecular weight component. Both chromium forms can be released from erythrocytes spontaneously or due to toxic effects.
Area of Science:
- Hematology
- Toxicology
- Biochemistry
Background:
- Human erythrocytes (red blood cells) can accumulate radioactive chromium.
- Chromium in erythrocytes exists in at least two distinct molecular forms.
- These forms have different properties and behaviors within the cell.
Purpose of the Study:
- To characterize the different forms of radioactive chromium within human erythrocytes.
- To investigate the release mechanisms of these chromium forms from red blood cells.
- To understand the implications of spontaneous and induced release.
Main Methods:
- Utilizing radioactive chromium (e.g., 51Cr) for tracking.
- Cellular fractionation to separate macromolecular-bound and low molecular weight forms.
- Incubation of erythrocytes under normal and toxic conditions.
- Measurement of chromium release from cells over time.
Main Results:
- Radioactive chromium was identified in two main fractions: bound to macromolecules (like hemoglobin) and in a low molecular weight form.
- Both macromolecular-bound and low molecular weight chromium were observed to be released from erythrocytes.
- Release occurred both spontaneously during normal cell conditions and was accelerated by toxic induction.
Conclusions:
- Erythrocytes contain radioactive chromium in at least two distinct molecular forms.
- The release of these chromium forms from red blood cells is a significant cellular process.
- Toxicological factors can influence and potentially increase the rate of chromium release from erythrocytes.
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