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pp60c-src encoded by the proto-oncogene c-src is a product of sensory neurons

P F Maness

    Journal of Neuroscience Research
    |January 1, 1986
    PubMed

    Insights

    Proto-oncogenes like pp60c-src are found in animal cells. This study shows pp60c-src is expressed in sensory neurons of the peripheral nervous system, specifically in chick dorsal root ganglia.

    Area of Science:

    • Molecular Biology
    • Neuroscience
    • Cell Biology

    Background:

    • Proto-oncogenes are homologous to retroviral transforming genes.
    • pp60c-src is a protein kinase encoded by a proto-oncogene with unknown function.
    • pp60c-src is preferentially synthesized in brain and neural retina.

    Purpose of the Study:

    • To investigate the expression of pp60c-src in the peripheral nervous system.
    • To determine if pp60c-src is present in sensory neurons.

    Main Methods:

    • Used antisera against Rous sarcoma virus transforming protein (pp60v-src) to detect pp60c-src.
    • Employed Western immunoblotting to analyze protein levels in chick embryo dorsal root ganglia.
    • Utilized immunoperoxidase staining and indirect double immunofluorescence on cultured sensory neurons.

    Main Results:

    • Elevated levels of pp60c-src were detected in dorsal root ganglia of stage 30 chick embryos.
    • pp60c-src immunoreactivity was observed in cells with neuronal morphology, not fibroblastic cells.
    • 96% of neurofilament-positive cells expressed pp60c-src, confirming its presence in sensory neurons.

    Conclusions:

    • pp60c-src is a neuronal protein.
    • pp60c-src is expressed in sensory neurons of the peripheral nervous system.
    • The study demonstrates pp60c-src expression in cultured sensory neurons.

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